POLR2E Rabbit Monoclonal Antibody [Clone ID: R05-8J5]

CAT#: TA385004

RNA Polymerases I/II/III Subunit ABC1 Rabbit monoclonal Antibody

Product Images

Western blot analysis of POLR2E in K562, C6, 3T3, Hela lysates using RNA Polymerases I/II/III Subunit ABC1 antibody.

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  Immunohistochemistry analysis of paraffin-embedded Human breast cancer using POLR2E antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Specifications

Product Data

• Clone Name: R05-8J5
• Applications: IHC, WB
• Recommended Dilution: WB: 1/2000
  IHC: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human POLR2E
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 25 kDa; Observed MW: 25 kDa
• Gene Name: polymerase (RNA) II subunit E
• Database Link:
  Entrez Gene 5434 Human
  UniProt ID P19388
• Background: Swiss-Prot Acc.P19388.DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process .
• Synonyms: hRPB25; hsRPB5; RPABC1; RPB5; XAP4