PI 3 Kinase p85 alpha (PIK3R1) Mouse Monoclonal Antibody [Clone ID: 1C8-2H4-9D9]

CAT#: TA384981

PI3 Kinase p85 alpha (1C8) Mouse monoclonal Antibody

Product Images

Western blot detection of PI3 Kinase p85α (1C8) in Hela,A549,HL-60,U2OS,C6 cell lysates using PI3 Kinase p85α (1C8) antibody. Observed band size:85kDa.

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  Western blot analysis of PI3 Kinase p85 alpha (1C8) in Hela, A549, HL-60, U2OS, C6 lysates using PI3 Kinase p85 alpha (1C8) antibody.

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  Immunohistochemistry analysis of paraffin-embedded rat Liver Tissue using PI3 Kinase p85 alpha (1C8) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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  Immunohistochemical analysis of paraffin-embedded Human tonsils using PI3 Kinase p85 alpha (1C8) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Specifications

Product Data

• Clone Name: 1C8-2H4-9D9
• Applications: IHC, WB
• Recommended Dilution: WB: 1/1000-2000
  IHC: 1/100-200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant protein expressed in E.coli.
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 85kDa
• Gene Name: phosphoinositide-3-kinase regulatory subunit 1
• Database Link:
  Entrez Gene 5295 Human
  UniProt ID P27986
• Background: Swiss-Prot Acc.P27986.Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).
• Synonyms: GRB1; p85; p85-ALPHA; PI3K