NEK9 Rabbit Monoclonal Antibody [Clone ID: R03-1F1]

CAT#: TA384852

NEK9 Rabbit monoclonal Antibody

Product Images

Western blot analysis of NEK9 in rat skeletal muscle lysates using NEK9 antibody.

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  Western blot analysis of NEK9 in Hela lysates using NEK9 antibody

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  Immunohistochemistry analysis of paraffin-embedded Human colon cancer using NEK9 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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  Immunocytochemistry analysis of NEK9 (green) in HT-1080 using NEK9 antibody,and DAPI(blue).

Specifications

Product Data

• Clone Name: R03-1F1
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/2000
  IHC: 1/20
  ICC/IF: 1/50
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human NEK9
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 107 kDa; Observed MW: 107 kDa
• Gene Name: NIMA related kinase 9
• Database Link:
  Entrez Gene 91754 Human
  UniProt ID Q8TD19
• Background: Swiss-Prot Acc.Q8TD19.Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.
• Synonyms: DKFZp434D0935; KIAA1995; MGC16714; MGC138306; Nek8; NERCC; NERCC1