G3BP (G3BP1) Rabbit Monoclonal Antibody [Clone ID: R06-5D2]

CAT#: TA384274

G3BP Rabbit monoclonal Antibody

Product Images

Western blot analysis of G3BP in K562, C6, 3T3, Hela lysates using G3BP antibody.

• 

  Immunohistochemistry of G3BP in paraffin-embedded Human colon cancer tissue using G3BP Rabbit mAb at dilution 1/20

• 

  Immunocytochemistry of G3BP (green) in Hela cells using G3BP Rabbit mAb at dilution 1/5, and DAPI(blue)

Specifications

Product Data

• Clone Name: R06-5D2
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/20
  ICC/IF: 1/20
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human G3BP
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 52 kDa; Observed MW: 68 kDa
• Gene Name: G3BP stress granule assembly factor 1
• Database Link:
  Entrez Gene 10146 Human
  UniProt ID Q13283
• Background: Swiss-Prot Acc.Q13283.ATP- and magnesium-dependent helicase (PubMed:9889278). Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends (PubMed:9889278). Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency (PubMed:9889278). Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA (PubMed:9889278). Phosphorylation-dependent sequence-specific endoribonuclease in vitro (PubMed:11604510). Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR (PubMed:11604510). May be a regulated effector of stress granule assembly (PubMed:12642610, PubMed:20180778).
• Synonyms: G3BP; G3BP-1; HDH-VIII; MGC111040