EIF4E Rabbit Monoclonal Antibody [Clone ID: R04-5A4]

CAT#: TA384157

Phospho-eIF4E (Ser209) Rabbit monoclonal Antibody

Product Images

Western blot detection of eIF4E (Phospho-Ser209) in K562,Rat Brain,C6,3T3 cell lysates using eIF4E (Phospho-Ser209) Rabbit mAb(1:1000 diluted).Predicted band size:25kDa.Observed band size:25kDa.

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  Immunohistochemistry of Phospho-eIF4E (Ser29) in paraffin-embedded Human lung cancer tissue using Phospho-eIF4E (Ser29) Rabbit mAb at dilution 1/5

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  Immunocytochemistry of eIF4E (Phospho-Ser209)(green) in Hela cells using eIF4E (Phospho-Ser209) Rabbit mAb at dilution 1/50, and DAPI(blue)

Specifications

Product Data

• Clone Name: R04-5A4
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000-1/10000
  IHC: 1/50
  ICC/IF: 1/100
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser209 of human eIF4E (Phosphorylated)
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 25 kDa; Observed MW: 25 kDa
• Gene Name: eukaryotic translation initiation factor 4E
• Database Link:
  Entrez Gene 1977 Human
  UniProt ID P06730
• Background: Swiss-Prot Acc.P06730.Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
• Synonyms: CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F; MGC111573