beta Catenin (CTNNB1) Rabbit Monoclonal Antibody [Clone ID: R07-9D5]

CAT#: TA384059

beta Catenin Rabbit monoclonal Antibody

Product Images

Western blot detection of beta Catenin in NTERA-2,3T3 lysates using beta Catenin antibody.Predicted band size:36kDa.Observed band size:86kDa.

• 

  Western blot detection of beta Catenin in C6 cell lysates using beta Catenin Rabbit mAb(1:1000 diluted).Predicted band size:86kDa.Observed band size:86kDa.

• 

  Immunocytochemistry analysis of beta Catenin (green) in 293T using beta Catenin antibody,and DAPI(blue).

Specifications

Product Data

• Clone Name: R07-9D5
• Applications: IF, WB
• Recommended Dilution: WB: 1/1000
  ICC/IF: 1/50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human beta Catenin
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 86 kDa; Observed MW: 86 kDa
• Gene Name: catenin beta 1
• Database Link:
  Entrez Gene 1499 Human
  UniProt ID P35222
• Background: Swiss-Prot Acc.P35222.Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and inTinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle .
• Synonyms: beta-catenin; CTNNB; DKFZp686D02253; FLJ25606; FLJ37923; OTTHUMP00000209288