Caspase 3 (CASP3) Rabbit Monoclonal Antibody [Clone ID: R05-2D6]

CAT#: TA383876

Cleaved-Caspase 3 p12 Rabbit monoclonal Antibody

Product Images

Western blot analysis of Caspase3 p12 in K562, 3T3, Hela lysates using Cleaved-Caspase 3 p12 antibody.

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  Immunohistochemical of Cleaved-Caspase-3 p12 in Human tonsil tissue using Cleaved-Caspase-3 p12 antibody at dilution 1/50

Specifications

Product Data

• Clone Name: R05-2D6
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/1000
  ICC/IF: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant protein of human Caspase-3 p12 (Cleaved)
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 32 kDa; Observed MW: 32,12 kDa
• Gene Name: caspase 3
• Database Link:
  Entrez Gene 836 Human
  UniProt ID P42574
• Background: Swiss-Prot Acc.P42574.Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
• Synonyms: apopain; CASP-3; CPP-32; CPP32; CPP32B; procaspase3; SCA-1; Yama