BCL2 Rabbit Monoclonal Antibody [Clone ID: R07-2A1]

CAT#: TA383812

Bcl2 Rabbit monoclonal Antibody

Product Images

Western blot detection of Bcl-2 in MCF-7 cell lysates using Bcl-2 Rabbit mAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:26KDa.

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  Western blot detection of Bcl-2 in K562,CHO-K1,3T3,Hela cell lysates using Bcl-2 Rabbit mAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:26KDa.

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  Immunohistochemistry of Bcl-2 in paraffin-embedded Human breast cancer tissue using Bcl-2 Rabbit mAb at dilution 1/1

Specifications

Product Data

• Clone Name: R07-2A1
• Applications: IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/20-1/500
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant protein of human Bcl-2
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 26 kDa; Observed MW: 26 kDa
• Gene Name: B-cell CLL/lymphoma 2
• Database Link:
  Entrez Gene 596 Human
  UniProt ID P10415
• Background: Swiss-Prot Acc.P10415.Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
• Synonyms: Bcl-2; OTTHUMP00000163680