ATP6V0D1 Rabbit Monoclonal Antibody [Clone ID: R07-1A5]

CAT#: TA383786

ATP6V0D1 Rabbit monoclonal Antibody

Product Images

Western blot analysis of ATP6V0D1 in K562, rat Brain, C6, 3T3, Hela lysates using ATP6V0D1 antibody.

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  Immunohistochemistry analysis of paraffin-embedded Human colon cancer using ATP6V0D1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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  Immunocytochemistry analysis of ATP6V0D1(green) in Hela using ATP6V0D1 antibody,and DAPI(blue)

Specifications

Product Data

• Clone Name: R07-1A5
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/20
  ICC/IF: 1/50
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant protein of human ATP6V0D1
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 40 kDa; Observed MW: 40 kDa
• Gene Name: ATPase H+ transporting V0 subunit d1
• Database Link:
  Entrez Gene 9114 Human
  UniProt ID P61421
• Background: Swiss-Prot Acc.P61421.Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis . May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium . In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633).
• Synonyms: ATP6D; ATP6DV; P39; VATX; Vma6; VPATPD