ATF2 Rabbit Monoclonal Antibody [Clone ID: R07-3E5]

CAT#: TA383758

Phospho-ATF2 (Thr71) Rabbit monoclonal Antibody

Product Images

Western blot analysis of Phospho-ATF2 (Thr71) in 3T3, Hela lysates using Phospho-ATF2 (Thr71) antibody.

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  Immunocytochemistry analysis of Phospho-ATF2 (Thr71) (green) in U87-MG using Phospho-ATF2 (Thr71) antibody,and DAPI(blue).

Specifications

Product Data

• Clone Name: R07-3E5
• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/1000
  ICC/IF: 1/100
  IP: 1/20
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF2 (Phosphorylated)
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 55 kDa; Observed MW: 70 kDa
• Gene Name: activating transcription factor 2
• Database Link:
  Entrez Gene 1386 Human
  UniProt ID P15336
• Background: Swiss-Prot Acc.P15336.Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
• Synonyms: CRE-BP1; CREB-2; CREB2; CREBP1; HB16; MGC111558; TREB7