AKT3 Rabbit Polyclonal Antibody

CAT#: TA383694

AKT Rabbit polyclonal Antibody

Product Images

Western blot analysis of AKT in K562, A549 and Jurkat lysates using AKT antibody.

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  Immunohistochemistry analysis of paraffin-embedded Human testis using AKT antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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  Immunofluorescence analysis of AKT in MCF-7 using AKT antibody.

Specifications

Product Data

• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/50-1/200
  ICC/IF: 1/50-1/200
  IP: 1/50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human AKT1/2/3
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 56kDa
• Gene Name: AKT serine/threonine kinase 3
• Database Link:
  Entrez Gene 10000 Human
  UniProt ID P31749
• Background: Swiss-Prot Acc.P31749,P31751,Q9Y243.Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
• Synonyms: DKFZp434N0250; OTTHUMP00000037911; OTTHUMP00000037912; PKB-GAMMA; PKBG; PRKBG; RAC-gamma; RAC-PK-gamma; STK-2