AMPK alpha 1 (PRKAA1) Rabbit Polyclonal Antibody

CAT#: TA383644

Phospho-AMPK alpha 1/2 (Thr183/Thr172) Rabbit polyclonal Antibody

Product Images

Western blot analysis of Phospho-AMPK alpha 1/2 (Thr183/Thr172) in various lysates using Phospho-AMPK alpha 1/2 (Thr183/Thr172) antibody.

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  Western blot analysis of Phospho-AMPK alpha 1/2 (Thr183/Thr172) in KB lysates using Phospho-AMPKα1/2 (T183/17, 2) antibody.

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  Western blot analysis of Phospho-AMPK alpha 1/2 in rat muscle heart using Phospho-AMPK alpha 1/2 antibody.

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  Western blot analysis of Phospho-AMPK alpha 1/2 in rat muscle lysates using Phospho-AMPK alpha 1/2 antibody.

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  Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-AMPK alpha 1/2 (Thr183/Thr172) antibody.High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody. was preabsorbed by immunogen peptide.

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  Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-AMPK alpha 1/2 (Thr183/Thr172) antibody.High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.Negetive contrl (right) obtaned from antibody was preabsorbed by immunogen peptide.

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Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: IF: 1/50-200
  WB: 1/500-1/2000
  IHC: 1/100-1/300
  ELISA: 1/40000
• Reactivities: Human, Mouse, Rat, Monkey, Pig
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189 (Phosphorylated)
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):63
• Gene Name: protein kinase AMP-activated catalytic subunit alpha 1
• Database Link:
  Entrez Gene 5562 Human
  UniProt ID Q13131
• Background: Swiss-Prot Acc.Q13131/P54646.Protein kinase AMP-activated catalytic subunit alpha 1 encoded by PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Synonyms: AMPK; AMPK1; AMPKa1; MGC33776; MGC57364