beta III Tubulin (TUBB3) Rabbit Polyclonal Antibody

CAT#: TA382932

βIII-Tubulin Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and βIII-Tubulin/β3-Tubulin Rabbit pAb knockout (KO) HeLa cells, using βIII-Tubulin/β3-Tubulin Rabbit pAb antibody (TA382932) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Western blot analysis of extracts of various cell lines, using βIII-Tubulin/β3-Tubulin antibody (TA382932) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human βIII-Tubulin/β3-Tubulin.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42kDa/50kDa
• Gene Name: tubulin beta 3 class III
• Database Link:
  Entrez Gene 10381 Human
  UniProt ID Q13509
• Background: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.
• Synonyms: beta-4; MC1R; TUBB4