Tuberin (TSC2) Rabbit Polyclonal Antibody

CAT#: TA382886

Phospho-TSC2-T1462 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of MCF7 cells, using Phospho-TSC2-T1462 antibody (TA382886) at 1:1000 dilution.MCF7 cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse
• Modifications: Phospho T1462
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around T1462 of human TSC2 (NP_000539.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 25kDa/187-200kDa
• Gene Name: tuberous sclerosis 2
• Database Link:
  Entrez Gene 7249 Human
  UniProt ID P49815
• Background: Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Synonyms: FLJ43106; LAM; TSC4; tuberin