Cardiac Troponin I (TNNI3) Rabbit Polyclonal Antibody

CAT#: TA382679

TNNI3 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using TNNI3 antibody (TA382679) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 5s.

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  Immunofluorescence analysis of mouse heart cells using TNNI3 antibody (TA382679) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human TNNI3 (NP_000354.4).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 24kDa
• Gene Name: troponin I3, cardiac type
• Database Link:
  UniProt ID P19429
• Background: Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
• Synonyms: CMD2A; CMH7; cTnI; MGC116817; RCM1; TNNC1