mtTFA (TFAM) Rabbit Polyclonal Antibody

CAT#: TA382445

TFAM Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using TFAM antibody (TA382445) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunofluorescence analysis of NIH/3T3 cells using TFAM Rabbit pAb (TA382445) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using TFAM Rabbit pAb (TA382445) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Chromatin immunoprecipitation analysis of extracts of K-562 cells, using TFAM antibody (TA382445) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Specifications

Product Data

• Applications: ChIP, ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  ChIP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TFAM (NP_003192.1).
• Formulation: Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 25kDa/29kDa
• Gene Name: transcription factor A, mitochondrial
• Database Link:
  Entrez Gene 7019 Human
  UniProt ID Q00059
• Background: This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants.
• Synonyms: MTTF1; MtTF1; MTTFA; mtTFA; OTTHUMP00000019633; TCF6; TCF6L1; TCF6L2; TCF6L3