STAT5A Rabbit Polyclonal Antibody

CAT#: TA382100

Phospho-STAT5-Y694 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of K562 cells, using Phospho-STAT5-Y694 pAb (TA382100) at 1:1000 dilution.K-562 cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Western blot analysis of extracts of A-431 cells, using Phospho-STAT5-Y694 pAb (TA382100) at 1:1000 dilution or STAT5B antibody (A12356).A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 60s.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:100 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho Y694
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A phospho specific peptide corresponding to residues surrounding Y694 of human STAT5.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 90kDa
• Gene Name: signal transducer and activator of transcription 5A
• Database Link:
  Entrez Gene 6776 Human
  UniProt ID P42229
• Background: Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
• Synonyms: MGF; STAT5