SHP1 (PTPN6) Rabbit Polyclonal Antibody

CAT#: TA380538

Phospho-SHP1-Y564 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HeLa and RAW 264.7 cells, using Phospho-SHP1-Y564 antibody (TA380538) at 1:2000 dilution. HeLa cell lysates were treated by CIP at 37°C for 1 hour.RAW 264.7 cell lysates were treated by CIP at 37°C for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit .|Exposure time: 3min.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse
• Modifications: Phospho Y564
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around Y564 of human PTPN6 (NP_002822.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 63kDa/67kDa/70kDa
• Gene Name: protein tyrosine phosphatase, non-receptor type 6
• Database Link:
  Entrez Gene 5777 Human
  UniProt ID P29350
• Background: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.
• Synonyms: HCP; HCPH; HPTP1C; PTP-1C; PTP1C; SH-PTP1; SHP-1; SHP-1L; SHP1