COX2 (PTGS2) Rabbit Polyclonal Antibody

CAT#: TA380507

COX2/PTGS2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of RAW264.7 cells, using COX2/PTGS2 antibody (TA380507) at 1:1000 dilution.Raw264.7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Western blot analysis of extracts of various cell lines, using COX2/PTGS2 antibody (TA380507) at 1:1000 dilution.THP-1 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:1000 - 1:4000
  IHC,1:50 - 1:200
  IF,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 375-604 of human COX2/PTGS2 (NP_000954.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 68kDa
• Gene Name: prostaglandin-endoperoxide synthase 2
• Database Link:
  Entrez Gene 5743 Human
  UniProt ID P35354
• Background: Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
• Synonyms: COX-2; COX2; Cyclooxygenase-2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; PHS-2