PSMC3 Rabbit Polyclonal Antibody

CAT#: TA380458

PSMC3 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PSMC3 antibody (TA380458) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.

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  Immunofluorescence analysis of U2OS cells using PSMC3 antibody (TA380458). Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug PSMC3 antibody (TA380458). Western blot was performed from the immunoprecipitate using PSMC3 antibody (TA380458) at a dilition of 1:1000.

Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
  IP,1:50 - 1:100
• Reactivities: Human
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PSMC3 (NP_002799.3).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 49kDa
• Gene Name: proteasome 26S subunit, ATPase 3
• Database Link:
  Entrez Gene 5702 Human
  UniProt ID P17980
• Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.
• Synonyms: MGC8487; TBP-1; TBP1