PRAF1 (POLR1E) Rabbit Polyclonal Antibody

CAT#: TA380135

POLR1E Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using POLR1E antibody (TA380135) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug POLR1E antibody (TA380135). Western blot was performed from the immunoprecipitate using POLR1E antibody (TA380135) at a dilition of 1:1000.

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IP,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human POLR1E (NP_071935.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 47kDa/53kDa
• Gene Name: polymerase (RNA) I subunit E
• Database Link:
  Entrez Gene 64425 Human
  UniProt ID Q9GZS1
• Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.
• Synonyms: FLJ13390; FLJ13970; PAF53; PRAF1; RP11-405L18.3