PLCG 2 (PLCG2) Rabbit Polyclonal Antibody

CAT#: TA380021

Phospho-PLC gamma 2 (PLCG2)-Y1217 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of Raji cells, using Phospho-PLC gamma 2 (PLCG2)-Y1217 antibody (TA380021) at 1:1000 dilution or PLC gamma 2 (PLCG2) antibody (A2182). Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 1s.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
• Reactivities: Human, Mouse
• Modifications: Phospho Y1217
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around Y1217 of human PLC gamma 2 (PLCG2) (NP_002652.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 147kDa
• Gene Name: phospholipase C gamma 2
• Database Link:
  Entrez Gene 5336 Human
  UniProt ID P16885
• Background: The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
• Synonyms: PLC-gamma-2; PLC-IV