Phospholipase A2 (PLA2G4A) Rabbit Polyclonal Antibody

CAT#: TA379998

Phospho-PLA2G4A-S505 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PLA2G4A-S505 antibody (TA379998) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 10s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho S505
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around of human Phospho-PLA2G4A-S505.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 85kDa
• Gene Name: phospholipase A2 group IVA
• Database Link:
  Entrez Gene 5321 Human
  UniProt ID P47712
• Background: This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants.
• Synonyms: CPLA2; cPLA2-alpha; lysophospholipase; MGC126350; PLA2G4