PI 3 Kinase p85 alpha (PIK3R1) Rabbit Polyclonal Antibody

CAT#: TA379941

Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HeLa cells, using Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 antibody (TA379941) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 120s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho Y467/Y199/Y464
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around Y467/Y199/Y464 of human p85-alpha (NP_852664.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42-54kDa/81-84kDa
• Gene Name: phosphoinositide-3-kinase regulatory subunit 1
• Database Link:
  Entrez Gene 5295 Human
  UniProt ID P27986
• Background: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
• Synonyms: GRB1; p85; p85-ALPHA; PI3K