PCMT1 Rabbit Polyclonal Antibody

CAT#: TA379697

PCMT1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PCMT1 antibody (TA379697).|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunofluorescence analysis of HeLa cells using PCMT1 antibody (TA379697). Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug PCMT1 antibody (TA379697). Western blot was performed from the immunoprecipitate using PCMT1 antibody (TA379697) at a dilition of 1:1000.

Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:100
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 62-286 of human PCMT1 (NP_001238978.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 24kDa
• Gene Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• Database Link:
  Entrez Gene 5110 Human
  UniProt ID P22061
• Background: This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Synonyms: OTTHUMP00000017400; PIMT