PAX6 Rabbit Polyclonal Antibody

CAT#: TA379655

PAX6 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PAX6 antibody (TA379655) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.

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  Immunofluorescence analysis of U-2 OS cells using PAX6 antibody (TA379655) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PAX6 (NP_000271.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 46kDa/48kDa
• Gene Name: paired box 6
• Database Link:
  Entrez Gene 5080 Human
  UniProt ID P26367
• Background: This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms.
• Synonyms: AN; AN2; D11S812E; MGC17209; MGDA; Oculorhombin; OTTHUMP00000038834; OTTHUMP00000038836; OTTHUMP00000038837; OTTHUMP00000038838; OTTHUMP00000038839; OTTHUMP00000038840; WAGR