NR1D1 Rabbit Polyclonal Antibody

CAT#: TA379282

Rev-Erbα/NR1D1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HepG2 cells, using Rev-Erbα/NR1D1 antibody (TA379282) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

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  Immunofluorescence analysis of NIH/3T3 cells using Rev-Erbα/NR1D1 Rabbit pAb (TA379282) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using Rev-Erbα/NR1D1 Rabbit pAb (TA379282) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human Rev-Erbα/Rev-Erbα/NR1D1.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 66kDa
• Gene Name: nuclear receptor subfamily 1 group D member 1
• Database Link:
  Entrez Gene 9572 Human
  UniProt ID P20393
• Background: This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes.
• Synonyms: ear-1; EAR1; hRev; Rev-erbA-alpha; Rev-ErbAalpha; THRA1; THRAL