MTOR Rabbit Polyclonal Antibody

CAT#: TA378784

mTOR Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using mTOR antibody (TA378784) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Western blot analysis of extracts of C6 cells, using mTOR antibody (TA378784) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of HeLa cells using mTOR antibody (TA378784) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC12 cells using mTOR antibody (TA378784) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of C6 cells using MTOR antibody (TA378784) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH/3T3 cells using MTOR antibody (TA378784) at dilution of 1:100. Blue: DAPI for nuclear staining.

• Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug mTOR antibody (TA378784). Western blot was performed from the immunoprecipitate using mTOR antibody (TA378784) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
  IP,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human mTOR (NP_004949.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 288kDa
• Gene Name: mechanistic target of rapamycin
• Database Link:
  Entrez Gene 2475 Human
  UniProt ID P42345
• Background: The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
• Synonyms: FLJ44809; FRAP; FRAP1; FRAP2; KIAA1303; RAFT1; RAPT1; RAPTOR; RPTOR