MEF2C Rabbit Polyclonal Antibody

CAT#: TA378450

Phospho-MEF2C-S396 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of K-562 and MCF-7 cells, using Phospho-MEF2C-S396 antibody (TA378450) at 1:1000 dilution. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. MCF7 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human
• Modifications: Phospho S396
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around S396 of human MEF2C (NP_002388.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 44kDa/47kDa/50kDa/51kDa/52kDa
• Gene Name: myocyte enhancer factor 2C
• Database Link:
  Entrez Gene 4208 Human
  UniProt ID Q06413
• Background: This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described.
• Synonyms: MEF2C