LAT Rabbit Polyclonal Antibody

CAT#: TA377962

Phospho-LAT-Y191 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Phospho-LAT-Y191 antibody (TA377962) at 1:1000 dilution.Jurkat cells were treated by CD-3 (10 ug/mL) at 37℃ for 2 minutes after serum-starvation overnight.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse
• Modifications: Phospho Y191
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A phospho specific peptide corresponding to residues surrounding Y191 of human LAT
• Formulation: Buffer: PBS with 0.02% sodium azide,1% BSA,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide,1% BSA,50% glycerol,pH7.3.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 24kDa/27kDa/28kDa
• Gene Name: linker for activation of T-cells
• Database Link:
  Entrez Gene 27040 Human
  UniProt ID O43561
• Background: The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Synonyms: LAT1; p36-38; pp36