LASP1 Rabbit Polyclonal Antibody

CAT#: TA377960

LASP1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using LASP1 antibody (TA377960) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

• 

  Immunofluorescence analysis of C6 cells using LASP1 Rabbit pAb (TA377960) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of L929 cells using LASP1 Rabbit pAb (TA377960) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of U-2 OS cells using LASP1 Rabbit pAb (TA377960) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• 

  Immunoprecipitation analysis of 300ug extracts of A-549 cells using 3ug LASP1 antibody (TA377960). Western blot was performed from the immunoprecipitate using LASP1 antibody (TA377960) at a dilition of 1:1000.

Show all 5 images

Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:1000 - 1:3000
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-205 of human LASP1 (NP_006139.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 23kDa/29kDa/36kDa
• Gene Name: LIM and SH3 protein 1
• Database Link:
  Entrez Gene 3927 Human
  UniProt ID Q14847
• Background: This gene encodes a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. The encoded protein is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. The encoded protein has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer.
• Synonyms: Lasp-1; MLN50