Cytokeratin 16 (KRT16) Rabbit Polyclonal Antibody

CAT#: TA377908

Cytokeratin 16 (KRT16) Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Cytokeratin 16 (KRT16) antibody (TA377908) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

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  Immunofluorescence analysis of A-549 cells using Cytokeratin 16 (KRT16) Rabbit pAb (TA377908) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of rat skin using Cytokeratin 16 (KRT16) Rabbit pAb (TA377908) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of mouse skin using Cytokeratin 16 (KRT16) Rabbit pAb (TA377908) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 190-430 of human Cytokeratin 16 (KRT16) (NP_005548.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 51kDa
• Gene Name: keratin 16
• Database Link:
  Entrez Gene 3868 Human
  UniProt ID P08779
• Background: The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus.
• Synonyms: CK-16; CK16; Cytokeratin-16; FNEPPK; K1CP; K16; Keratin-16; KRT16A; NEPPK