IRS1 Rabbit Polyclonal Antibody

CAT#: TA377578

IRS1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using IRS1 antibody (TA377578) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Western blot analysis of extracts of 293T cells, using IRS1 antibody (TA377578) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunofluorescence analysis of A-549 cells using IRS1 Rabbit pAb (TA377578) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of MCF7 cells using IRS1 Rabbit pAb (TA377578) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH/3T3 cells using IRS1 Rabbit pAb (TA377578) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using IRS1 Rabbit pAb (TA377578) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:1000
  IHC,1:50 - 1:100
  IF,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human IRS1
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 131kDa
• Gene Name: insulin receptor substrate 1
• Database Link:
  Entrez Gene 3667 Human
  UniProt ID P35568
• Background: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
• Synonyms: HIRS-1; IRS-1