Insulin (INS) Rabbit Polyclonal Antibody

CAT#: TA377522

Insulin Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of Rat pancreas, using Insulin antibody (TA377522) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.

• 

  Immunofluorescence analysis of Rat pancreas using Insulin Rabbit pAb (TA377522) at dilution of 1:100. Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of Mouse pancreatic islet using Insulin Rabbit pAb (TA377522) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human INS (NP_000198.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 11kDa/21kDa
• Gene Name: insulin
• Database Link:
  Entrez Gene 3630 Human
  UniProt ID P01308
• Background: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.
• Synonyms: ILPR; insulin; IRDN; OTTHUMP00000011162; OTTHUMP00000196038; proinsulin