GPLD1 Rabbit Polyclonal Antibody

CAT#: TA376713

GPLD1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using GPLD1 antibody (TA376713) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Western blot analysis of extracts of various cell lines, using GPLD1 antibody (TA376713) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of A-549 cells using GPLD1 Rabbit pAb (TA376713) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of HepG2 cells using GPLD1 Rabbit pAb (TA376713) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
  IF,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human GPLD1 (NP_001494.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 19kDa/92kDa
• Gene Name: glycosylphosphatidylinositol specific phospholipase D1
• Database Link:
  Entrez Gene 2822 Human
  UniProt ID P80108
• Background: Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane.
• Synonyms: GPIPLD; GPIPLDM; MGC22590; PIGPLD; PIGPLD1