GFAP Rabbit Polyclonal Antibody

CAT#: TA376544

GFAP Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using GFAP antibody (TA376544) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunofluorescence analysis of U-251 MG cells using GFAP Rabbit pAb (TA376544) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of rat brain using GFAP Rabbit pAb (TA376544) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of mouse brain using GFAP Rabbit pAb (TA376544) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 49kDa/50kDa
• Gene Name: glial fibrillary acidic protein
• Database Link:
  Entrez Gene 2670 Human
  UniProt ID P14136
• Background: This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Synonyms: FLJ45472