c Fos (FOS) Rabbit Polyclonal Antibody

CAT#: TA376319

Phospho-c-Fos-T232 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HepG2 cells, using Phospho-c-Fos-T232 antibody (TA376319) at 1:1000 dilution or c-Fos antibody (A17351).HepG2 cells were treated by 10% FBS for after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho T232
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around T232 of human c-Fos (NP_005243.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 28kDa/36kDa/40kDa
• Gene Name: Fos proto-oncogene, AP-1 transcription factor subunit
• Database Link:
  Entrez Gene 2353 Human
  UniProt ID P01100
• Background: The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
• Synonyms: AP-1; C-FOS; G0S7