Filamin A (FLNA) Rabbit Polyclonal Antibody

CAT#: TA376274

Filamin A Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Filamin A antibody (TA376274) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Filamin A antibody (TA376274). Western blot was performed from the immunoprecipitate using Filamin A antibody (TA376274) at a dilition of 1:1000.

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IP,1:50 - 1:100
• Reactivities: Human, Mouse
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2348-2647 of human Filamin A (NP_001104026.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 280kDa
• Gene Name: filamin A
• Database Link:
  Entrez Gene 2316 Human
  UniProt ID P21333
• Background: The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: ABP-280; ABPX; Alpha-filamin; DKFZp434P031; Filamin-1; FLN; FLN1; FMD; MNS; NHBP; OPD; OPD1; OPD2