Ezrin (EZR) Rabbit Polyclonal Antibody

CAT#: TA376053

Ezrin Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Ezrinin antibody (TA376053) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunofluorescence analysis of C6 cells using Ezrinin antibody (TA376053) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using Ezrinin antibody (TA376053) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Ezrinin antibody (TA376053). Western blot was performed from the immunoprecipitate using Ezrinin antibody (TA376053) at a dilition of 1:1000.

Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:100
  IP,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human Ezrinin (NP_003370.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 69kDa
• Gene Name: ezrin
• Database Link:
  Entrez Gene 7430 Human
  UniProt ID P15311
• Background: The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
• Synonyms: CVIL; CVL; Cytovillin; DKFZp762H157; ezrin; FLJ26216; MGC1584; p81; VIL2; Villin-2