EIF4EBP1 Rabbit Polyclonal Antibody

CAT#: TA375832

Phospho-eIF4EBP1-S65 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of 293T cells, using Phospho-eIF4EBP1-S65 antibody (TA375832) at 1:1000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IP,1:50 - 1:100
• Reactivities: Human
• Modifications: Phospho S65
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around S65 of human Phospho-eIF4EBP1-S65 (NP_004086.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 12kDa
• Gene Name: eukaryotic translation initiation factor 4E binding protein 1
• Database Link:
  Entrez Gene 1978 Human
  UniProt ID Q13541
• Background: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
• Synonyms: 4E-BP1; 4EBP1; BP-1; MGC4316; PHAS-I