elF2 alpha (EIF2S1) Rabbit Polyclonal Antibody

CAT#: TA375800

Phospho-eIF2α-S51 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HeLa cells, using Phospho-eIF2α-S51 pAb (TA375800) at 1:2000 dilution or EIF2S1 antibody. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2α-S51 pAb (TA375800). Western blot was performed from the immunoprecipitate using Phospho-eIF2α-S51 pAb (TA375800) at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.

Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:1000 - 1:2000
  IHC,1:50 - 1:200
  IP,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho S51
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around S51 of human eIF2α (NP_004085.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 36kDa
• Gene Name: eukaryotic translation initiation factor 2 subunit alpha
• Database Link:
  Entrez Gene 1965 Human
  UniProt ID P05198
• Background: The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
• Synonyms: EIF-2; eIF-2-alpha; EIF-2A; EIF-2alpha; EIF2; EIF2A