EEF2 Rabbit Polyclonal Antibody

CAT#: TA375728

Phospho-eEF2-T56 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Phospho-eEF2-T56 antibody (TA375728) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight. C6 cells were treated by Forskolin (10uM) for 1 hour after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 5s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho T56
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around T56 of human Phospho-eEF2-T56 (NP_001952.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 95kDa
• Gene Name: eukaryotic translation elongation factor 2
• Database Link:
  Entrez Gene 1938 Human
  UniProt ID P13639
• Background: This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
• Synonyms: EEF-2; EF-2; EF2