DNMT1 Rabbit Polyclonal Antibody

CAT#: TA375561

DNMT1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of 293T cells, using DNMT1 antibody (TA375561) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 3s.

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  Western blot analysis of extracts of HeLa cells, using DNMT1 antibody (TA375561) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 3s.

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  Western blot analysis of extracts of various cell lines, using DNMT1 antibody (TA375561) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Confocal immunofluorescence analysis of HeLa cells using DNMT1 Polyclonal Antibody (TA375561) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Confocal immunofluorescence analysis of HeLa cells using DNMT1 Polyclonal Antibody (TA375561) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug DNMT1 antibody (TA375561). Western blot was performed from the immunoprecipitate using DNMT1 antibody (TA375561) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human DNMT1.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 144kDa/183kDa/184kDa
• Gene Name: DNA (cytosine-5-)-methyltransferase 1
• Database Link:
  Entrez Gene 1786 Human
  UniProt ID P26358
• Background: This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
• Synonyms: AIM; CXXC9; DNMT; FLJ16293; M.HsaI; MCMT; MGC104992