Cullin 4B (CUL4B) Rabbit Polyclonal Antibody

CAT#: TA375233

CUL4B Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CUL4B antibody (TA375233) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

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  Immunofluorescence analysis of U2OS cells using CUL4B antibody (TA375233). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.

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  Immunofluorescence analysis of U2OS cells using CUL4B antibody (TA375233).

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CUL4B (NP_003579.3).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 84kDa/102kDa/103kDa
• Gene Name: cullin 4B
• Database Link:
  Entrez Gene 8450 Human
  UniProt ID Q13620
• Background: This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: CUL-4B; cullin-4B; DKFZp686F1470; KIAA0695; MRXHF2; MRXSC; SFM2