COMT Rabbit Polyclonal Antibody

CAT#: TA374983

COMT Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using COMT antibody (TA374983) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

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  Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug COMT antibody (TA374983). Western blot was performed from the immunoprecipitate using COMT antibody (TA374983) at a dilition of 1:1000.

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IP,1:50 - 1:100
• Reactivities: Human, Mouse
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-221 of human COMT (NP_009294.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 24kDa/30kDa
• Gene Name: catechol-O-methyltransferase
• Database Link:
  Entrez Gene 1312 Human
  UniProt ID P21964
• Background: Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
• Synonyms: catechol-O-methyltransferase; OTTHUMP00000197749; OTTHUMP00000197750