Caspase 3 (CASP3) Rabbit Polyclonal Antibody

CAT#: TA374281

Caspase-3 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and Caspase-3 knockout (KO) 293T cells, using Caspase-3 antibody (TA374281) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Western blot analysis of extracts of Jurkat cells, using Caspase-3 antibody (TA374281) at 1:1000 dilution.Jurkat cells were treated by staurosporine(1 uM) for 3 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Caspase-3 Rabbit pAb (TA374281) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using [KO Validated] Caspase-3 Rabbit pAb (TA374281) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U2OS cells using [KO Validated] Caspase-3 Rabbit pAb (TA374281) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase-3 (NP_004337.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 31kDa
• Gene Name: caspase 3
• Database Link:
  Entrez Gene 836 Human
  UniProt ID P42574
• Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
• Synonyms: apopain; CASP-3; CPP-32; CPP32; CPP32B; procaspase3; SCA-1; Yama