BCAR1 Rabbit Polyclonal Antibody

CAT#: TA373952

Phospho-BCAR1-Y410 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HeLa cells, using Phospho-BCAR1-Y410 antibody (TA373952) at 1:5000 dilution. HeLa cells were treated by Pervanadate (1nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 5s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse
• Modifications: Phospho Y410
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A phospho specific peptide corresponding to residues surrounding Y410 of human BCAR1
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 77kDa/93kDa/95kDa/97kDa
• Gene Name: BCAR1, Cas family scaffolding protein
• Database Link:
  Entrez Gene 9564 Human
  UniProt ID P56945
• Background: BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009]
• Synonyms: CAS; CAS1; CASS1; CRKAS; FLJ12176; FLJ45059; P130Cas