RhoGAP (ARHGAP5) Rabbit Polyclonal Antibody

CAT#: TA373656

RhoGAP Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using RhoGAP antibody (TA373656) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

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  Immunofluorescence analysis of HeLa cells using RhoGAP antibody (TA373656) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1292-1501 of human RhoGAP (NP_001164.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 26kDa/27kDa/172kDa
• Gene Name: Rho GTPase activating protein 5
• Database Link:
  Entrez Gene 394 Human
  UniProt ID Q13017
• Background: Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: GFI2; p190-B; RhoGAP5