APE1 (APEX1) Rabbit Polyclonal Antibody

CAT#: TA373573

APEX1/APE1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and APEX1/APE1 knockout (KO) 293T cells, using APEX1/APE1 antibody (TA373573) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Western blot analysis of extracts of various cell lines, using APEX1/APE1 antibody (TA373573).|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.

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  Immunofluorescence analysis of HeLa cells using APEX1/APE1 antibody (TA373573). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of A549 cells using APEX1/APE1 antibody (TA373573).

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  Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug APEX1/APE1 antibody (TA373573). Western blot was performed from the immunoprecipitate using APEX1/APE1 antibody (TA373573) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:100
  IP,1:50 - 1:200
• Reactivities: Human
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1/APE1 (NP_542380.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 35kDa
• Gene Name: apurinic/apyrimidinic endodeoxyribonuclease 1
• Database Link:
  Entrez Gene 328 Human
  UniProt ID P27695
• Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.
• Synonyms: APE; APE-1; APE1; APEN; APEX; APX; HAP1; REF-1; REF1