ALKBH7 Rabbit Polyclonal Antibody

CAT#: TA373465

ALKBH7 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ALKBH7 antibody (TA373465) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:200 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human ALKBH7 (NP_115682.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 24kDa
• Gene Name: alkB homolog 7
• Database Link:
  Entrez Gene 84266 Human
  UniProt ID Q9BT30
• Background: May function as protein hydroxylase; can catalyze auto-hydroxylation at Leu-110 (in vitro, but this activity may be due to the absence of the true substrate. Required to induce programmed necrosis in response to DNA damage caused by cytotoxic alkylating agents. Acts by triggering the collapse of mitochondrial membrane potential and loss of mitochondrial function that leads to energy depletion and cell death. ALKBH7-mediated necrosis is probably required to prevent the accumulation of cells with DNA damage. Does not display DNA demethylase activity. Involved in fatty acid metabolism (By similarity.
• Synonyms: MGC10974; SPATA11; UNQ6002